CP04-100 – Liquid Cas9 Protein with NLS – 100 µg (20 µg x 5)

This liquid form Cas9 protein with nuclear localization signal (NLS) is pre-diluted to 1 mg/ml for immediate use in CRISPR microinjection workflows. Provided in five ready-to-use vials (20 µg each, 100 µg total), it delivers 600 pmol total (6 µM × 20 µl × 5). The pre-aliquoted format reduces handling time and supports consistent performance in high-precision genome editing.

Optimized for in vivo embryo editing in zebrafish, mice, or other model organisms, this format eliminates reconstitution steps, saving time and reducing variability. The NLS ensures nuclear localization, and the high-purity liquid formulation is compatible with sgRNA complexing for RNP formation.

This ready-to-inject Cas9 is ideal for researchers working with tight protocols, rapid turnaround needs, or automated microinjection systems.

• Ready-to-use liquid format (1 mg/ml)
• Eliminates reconstitution steps
• Dual NLS for efficient nuclear delivery
• Suitable for microinjection and embryo editing
• Five vials for flexible experimental use
• Compatible with sgRNA and RNP assembly workflows

• Direct injection into embryos (mouse, zebrafish, etc.)
• CRISPR RNP-based genome editing
• Knockout and knock-in via microinjection
• In vivo editing in early-stage development
• Fast-start gene editing workflows
• Time-sensitive or high-throughput CRISPR programs
• In vitro cleavage assay

• 5 × 20 µg liquid Cas9 protein with NLS (1 mg/ml)
• RNP formation instructions
• Microinjection compatibility guide
• Access to technical support for optimization

For help with setup, delivery, or experiment planning, contact our support team. We provide protocol templates, RNP ratios, and injection timing advice.