FAQ
Why should you order custom PNA oligomers from PNA Bio?
As a specialized PNA company, PNA Bio is dedicated to delivering high-quality products, offering >95% purity after HPLC purification and including MALDI-TOF mass data for all products.
Does PNA Bio provide a warranty for their products?
Yes, PNA Bio stands fully behind the quality of their products. If any quality issue arises, PNA Bio will provide a free replacement and, if necessary, a refund if the replacement also does not work.
If I order Cas9 protein today, when will I receive it?
All Cas9 protein products are in stock and shipped the same day if the order is placed by 5 pm ET, for next-day delivery.
Has the Cas9 protein from PNA Bio been used for embryo injection?
Yes, our Cas9 protein has been successfully used for embryo injection in several species, including mice, zebrafish, rats, Xenopus, butterflies, mosquitoes, C. elegans, and fruit flies.
Can PNA Bio's Cas9 protein be used for transfection to generate KO and KI cell lines?
Yes, our Cas9 protein includes two NLS for nuclear transport and is endotoxin-free, making it suitable for use in any cell type or embryo injection.
How is the Cas9 protein shipped, and how long is it stable?
Our lyophilized Cas9 protein is shipped at room temperature and should be stable for at least 2 weeks under these conditions.
Are the Cas9 proteins in lyophilized or liquid form?
All Cas9 protein products are lyophilized except for CP04 products.
What is the quality assurance for PNA oligomers synthesized by PNA Bio?
All PNA products are analyzed by HPLC for purity and by MALDI-TOF for mass confirmation, ensuring a thorough synthesis process. Most PNA probes are provided with >95% purity.
Does PNA Bio offer custom PNA conjugation to peptides or chemicals?
Yes, PNA Bio can conjugate peptides or other chemicals to PNA via amide linkage or SMCC ligation.
Can PNA Bio help design custom PNA against my targets?
Yes, the PNA Bio team can review your sequence or design PNA probes for your target at no additional charge.
How can labeled PNA be used to quantify siRNA for pharmacokinetics studies?
PNA conjugated with fluorescent dyes or biotin can capture short DNA or RNA targets like siRNA due to its high affinity. The resulting complex can then be analyzed by HPLC for quantification.
How soon can I receive PNA telomere probes?
All the PNA telomere and centromere probes are in stock and ship the same day if the order is placed by 5 pm ET, for next-day delivery.
Can PNA Bio provide a labeled PNA with a specific fluorophore that is not listed? Can it be labeled at the 5' or 3' end?
Most NHS ester-form fluorophores can be conjugated to PNA at either end. For 3′ end labeling, one lysine is added.
What is the maximum length of PNA that PNA Bio can synthesize?
PNA Bio can synthesize PNA up to approximately 50mer, depending on the sequence.
Why is PNA oligo superior to DNA for hybridization?
PNA’s backbone is composed of methylene carbonyl bonds, like peptides, and lacks charged phosphate groups, resulting in stronger binding to DNA due to reduced electrostatic repulsion. PNA is also resistant to DNases and proteases and remains stable across a wide pH and salt range.
How does a surrogate reporter system help to select recombination events?
Our reporter plasmid contains GFP out of frame, which becomes in frame through an active recombination event. By selecting cells with GFP expression, the chances of finding a cell with recombination improve by 10–50 fold.