Gamma/Bis PNA

Bis-PNA and Gamma-PNA for Sequence-Specific dsDNA Targeting

PNA Bio Inc. provides offers high-quality bis-PNA and gamma-PNA oligomers designed for efficient, sequence-specific binding to double-stranded DNA (dsDNA). These advanced PNA variants are powerful tools for applications such as gene targeting, genome editing, and molecular diagnostics.

🔹Bis-PNA: Strand Invasion via Triplex Formation

Bis-PNA is a specially engineered molecule composed of two homopyrimidine PNA strands connected by a flexible linker. This configuration allows the PNA to invade double-stranded DNA and form highly stable (PNA)₂/DNA triplexes via strand displacement, rather than conventional triple-helix binding.

  • One strand binds via Watson–Crick base pairing (anti-parallel to the DNA)
  • The second strand binds via Hoogsteen base pairing (parallel to the DNA)
  • Pseudoisocytosine (J) replaces cytosine in the Hoogsteen strand, enabling pH-independent binding

This unique structure allows bis-PNA to bind with high affinity and specificity to homopurine sequences in dsDNA under physiological conditions, without denaturation.

💡 Applications:

  • Gene targeting
  • Mutation detection
  • Molecular diagnostics
  • Sequence-specific genome editing

PNA Bioprovides fully customized bis-PNA probes, synthesized with high fidelity and rigorously quality-controlled for reliable performance.

🔹Gamma-PNA: Enhanced Affinity and Solubility

Gamma PNA is a next-generation PNA backbone modified at the gamma position with various side chains. This structural enhancement introduceschirality and conformational pre-organization, significantly improving:

  • Binding affinity (increase the Tm by 5–8°C per substitution)
  • Sequence specificity
  • Water solubility
  • Double-stranded DNA invasion capability

These features make gamma-PNA ideal for high-performance hybridization and targeting even under challenging conditions.

Gamma PNA for advanced molecular research – PNA Bio

🧬Available Gamma Modifications

Cysteine (γCys): Enables thiol-based conjugation

Mini-PEG gamma: Increases flexibility and aqueous solubility

 

📦Custom PNA Services

Whether you’re targeting a specific DNA sequence or need advanced modifications for specialized applications, PNA Bio is here to support your project.

📧 Contact us at info@pnabio.com or visit our Custom PNA Oligos page to get started.

  • Lysine (γLys): Enhances solubility, enables internal labeling, and may enhance cell permeability
  • Glutamic acid (γGlu): Adds negative charge and improves solubility
  • Alanine (γAla): Introduces hydrophobic character
  • Serine (γSer): Enhances solubility without affecting charge
  1. Nanopore-Based Target Sequence Detection. Morin TJ et al (2016)PLoS One 11(5):e0154426.
  2. Single-stranded γPNAs for in vivo site-specific genome editing viaWatson-Crick recognition. Bahal R et al (2015) Curr Gene Ther 14(5):331-342.
  3. Site-specific genome editing in PBMCs With PLGA nanoparticle-delivered PNAs confers HIV-1 resistance in humanized mice. Schleifman EB et al (2013) Mol Ther Nucleic Acids 2:e135.
  4. Sequence-unrestricted, Watson-Crick recognition of double helical B-DNA by (R)-miniPEG-γPNAs. Bahal R et al (2012) Chembiochem 13(1):56-60.
  5. Quadruplex formation is necessary for stable PNA invasion into duplex DNA of BCL2 promoter region. Onyshchenko MI et al (2011) Nuc Acids Res 39(16):7114-23.
  6. Strand-invasion of extended, mixed-sequence B-DNA by γPNAs. He G et al (2010) J Am Chem Soc 131(34):12088-90.
  7. PNA-dependent gene chemistry: stable coupling of peptides and oligonucleotidesto Plasmid DNA. Zelphati O et al (2000) Biotech 28(2)304-315.
  8. Efficient pH-independent sequence-specific DNA binding by pseudoisocytosine-containing bis-PNA. Egholm M et al (1995) Nuc Acid Res 23(2):217-111.

Custom PNA oligos provided by PNA Bio will be provided >95% purity, accompanied by COA including HPLC and mass data. Synthesis usually takes 3~4 weeks for bis PNA or gamma PNA.

The price of custom oligo is dependent on the length, amount, and label. Please indicate the specifics in the quote request. Feel free to check out PNA Tool for Tm calculation and other guidelines for PNA design.

Minimum amount is 50 nmole for non-labeled PNA and 25 nmole for labeled PNA.

Please send a quote request and order to order@pnabio.com or Contact us for inquire. We can also help the design of bis PNA or gamma PNA.