CP01-50 – Cas9 Protein with NLS – 50 µg

This Cas9 protein with NLS is a reliable reagent for CRISPR/Cas9 genome editing by microinjection. Delivered as 50 µg lyophilized powder, it reconstitutes to 1 mg/ml and provides 300 pmol total (6 µM × 50 µl). 

Engineered with dual nuclear localization signal (NLS), this Cas9 ensures efficient transport into the nucleus for successful double-strand DNA cleavage. Suitable for microinjection into zebrafish, mice, or rat embryos, it supports both knockout and knock-in model development. It is optimized for pairing with synthetic sgRNA to form highly efficient ribonucleoprotein (RNP) complexes for immediate delivery into cells and embryos.

• Includes dual NLS for rapid nuclear localization
• Provides 300 pmol of active Cas9 enzyme
• Reconstitutes quickly to 1 mg/ml
• Low endotoxin, high purity, nuclease-free
• Ready for immediate RNP complexing with sgRNA
• Gene editing for microinjection into embryos or transfection into cells 

• 50 µg lyophilized Cas9 protein with NLS
• Storage and reconstitution instructions
• RNP assembly and microinjection protocol
• Access to expert technical support
• Nuclease free water with 20% glycerol (can be replaced with Nuclease free water upon request)

• CRISPR embryo microinjection (mouse, zebrafish, rat)
• In vivo gene editing using direct RNP delivery
• Knockout and knock-in model generation
• In vitro cleavage assay
• Functional genetic screens in early-stage embryos

For help selecting delivery methods or setting up embryo injection, contact our support team for technical assistance and experiment design advice.