CP04-500 – Liquid Cas9 Protein with NLS – 500 µg (100 µg x 5)

This liquid Cas9 protein with nuclear localization signal (NLS) is a ready-to-use, high-purity reagent designed for large-scale CRISPR transfection workflows. Supplied as five 100 µg vials, it’s pre-diluted to 5 mg/ml and delivers a total of 3000 pmol active Cas9 enzyme (30 µM × 20 µl × 5). This format is perfect for labs needing fast, consistent, and scalable editing in cell lines or embryos.

The dual NLS enables direct nuclear localization after delivery, increasing CRISPR editing efficiency. Pre-formulated in liquid form, this Cas9 eliminates reconstitution steps, saving time while ensuring reproducibility across large experiments.

Validated for use with sgRNA in RNP format, this product is suitable for electroporation, lipofection, and nucleofection. It’s ideal for genome engineering in HEK293, iPSC, stem cells, and other difficult-to-transfect mammalian cell lines.

• Supplied in ready-to-use liquid form (5 mg/ml)
• 5 × 100 µg vials provide flexibility and scale
• Delivers 3000 pmol of Cas9 protein
• Dual NLS for efficient nuclear delivery
• Eliminates reconstitution steps
• Compatible with RNP workflows using synthetic sgRNA

• Genome editing using RNP transfection
• Knockout and knock-in gene editing
• High-throughput CRISPR cell engineering
• Functional screening and pathway studies
• iPSC and primary cell line editing
• CRISPR/Cas9 delivery via electroporation or lipofection

• 5 × 100 µg liquid Cas9 protein with NLS (5 mg/ml)
• RNP formation instructions
• sgRNA pairing recommendations
• Dedicated technical support

Need help optimizing delivery or scaling up experiments? Contact our team for expert support on transfection, sgRNA ratios, or batch planning.