PFB01 – PNA FISH Hybridization Buffer – 30 mL

This Cas9 reaction buffer is a 10X optimized formulation designed to support the in vitro activity of Cas9 proteins during DNA cleavage assays. With a precise blend of 50 mM Tris-HCl (pH 7.9), 100 mM NaCl, 10 mM MgCl₂, and 1 mM DTT, it ensures efficient and reproducible endonuclease activity in a controlled reaction environment.

Ideal for validating sgRNA sequences, confirming Cas9 activity, or setting up in vitro cleavage reactions, this buffer simplifies your lab workflow by eliminating the need to prepare custom buffers.

Supplied in a convenient 500 µl format, it can be diluted to 1X working concentration and used in various Cas9 protein-based assays including wild-type, nickase, or high-fidelity variants.

• Pre-formulated 10X buffer for Cas9 activity testing
• Eliminates need for custom buffer preparation
• Compatible with all Cas9 protein variants (WT, nickase, HF, dCas9)
• Stable and reliable for DNA cleavage validation
• Supports RNP and plasmid digestion reactions
• pH-balanced and quality controlled

• In vitro DNA cleavage using Cas9 proteins
• sgRNA sequence validation and activity checks
• Pre-screening of RNP complex efficiency
• Quality control assays for Cas9 delivery batches
• Educational demonstrations of CRISPR cleavage
• Troubleshooting low cleavage efficiency in RNP prep

• 500 µl of 10X Cas9 Reaction Buffer
• Usage and dilution instructions
• Access to troubleshooting and setup resources

Have questions about reaction setup or cleavage efficiency? Contact our support team for help with buffer use, DNA templates, or protein compatibility.