Custom sgRNA/Cas9
Custom sgRNA
High-Quality, Ready-to-Use Single Guide RNAs for Your CRISPR Applications
Custom sgRNA Synthesis Service
PNA Bio offers reliable, fast, and affordable custom sgRNA synthesis to support your CRISPR-Cas9 genome editing needs. Our guide RNAs are chemically synthesized with chemical modifications to make it more stable in vivo, sequence-verified, and delivered ready for use to form an RNP complex for transfection or microinjection. Whether you’re performing knockouts, knock-ins, or gene regulation experiments, our sgRNA synthesis service ensures high activity and specificity. We offer free design service.
Key Advantages
- complimentary gRNA design: Our technical support team selects the best gRNA using multiple parameters
- 2′-O-methyl modifications to increase gRNAstability, nuclease resistance, and decreases immune activation
- High Purity: Desalting (>60%) or HPLC purification (>90%)
- Fast Turnaround: Typical production time is 2 weeks
- Free technical support for your project
HEK293 cells are transfected with increasing amount of plasmid expressing sgRNA in the presence or absence of Cas9 plasmid. In/del caused by CRISPR/Cas9 complex was assessed by T7E1 assay.
Email your target gene to info@pnabio.com for more information.
For gRNA design, please check out our gRNA Design Tool.
Custom sgRNA Synthesis Service
Comprehensive synthesis service for CRISPR applications
- Includes: sgRNA design, off-target analysis, and chemical synthesis
- Formats: Lyophilized powder starting from 20 µg
- Purification options: Desalting (>60% purity) or HPLC (>90% purity)
- Injection/transfection ready
- Turnaround time: ~2 weeks
- Lowest price guaranteed
Custom sgRNA Vector Service
End-to-end sgRNA expression vector preparation
- Includes: sgRNA design, off-target search, and plasmid synthesis
- Transfection/injection ready
- Delivers sgRNA-Cas9 co-expression plasmid
- Promoter options: CMV or EF1α
- Optional reporter cassettes:
- GFP/HygroR
- RFP/PuroR
- Quality confirmed by agarose gel electrophoresis
- Turnaround time: ~2 weeks
sGFP Control sgRNA – AR04
Validated positive control to check Cas9 and gRNA activity
- Efficiently cleaves eGFP in vitro and in vivo
- Supplied as injection-ready lyophilized powder
- Also available with plasmid and Cas9 protein for assay setups
- Sizes available: 20 µg or 100 µg (5 × 20 µg tubes)
Surrogate Reporter Vectors
Tools to enrich genome editing events
- Validates guide RNA activity in cells
- Enables clone screening via FACS, MACS, or drug selection (hygromycin resistance)
- Options:
- Empty plasmid to clone in the gRNA targeting site(s)
- Custom plasmid with your nuclease target sequence
- Turnaround time: in stock for empty plasmids, 2 weeks for custom designs:
Learn more about Reporters for Nucleases page
- Laminar flow downregulates Notch activity to promote lymphatic sprouting. Choi D et al. (2017) J. Clin Investigation. In press.
- Generation and characterization of tamoxifen-inducible Pax9-CreER Knock-In Mice using CrispR/Cas9. Jifan F et al. (2016) Genesis 54(9):490-496.
- Efficient delivery of nuclease proteins for genome editing in human stem cells and primary cells. Liu J et al. (2015) Nat Protoc. 10:1842-1859.
- Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins. Kim S et al. (2013) Gen Res. 24:1012-1019.
- Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection. Liang X et al (2015) J Biotech. 208: 44-53.
Any inquires, please send us an email to info@pnabio.com for more information.
| Cat No | Description | Price |
|---|---|---|
| DR01 | Custom sgRNA/Cas9 expression plasmids | inquire |
| DR02 | Activity validated sgRNA/Cas9 vector by mismatch sensitive nuclease assay in cells | inquire |
| AR01 | Custom IVT sgRNA, injection ready (50 ug) | inquire |
| AR02 | 1~3 in vitro validated sgRNA, injection ready (50ug each) | inquire |
| AR04 | eGFP control sgRNA (20 ug or 100 ug) | inquire |
| Surrogate reporter system | ||
| RV01 | GFP surrogate reporter system / 5 ug | $350 |
| RV02 | MACS/GFP surrogate reporter system / 5 ug | $350 |
| RV03 | HygR/GFP surrogate reporter system / 5 ug | $350 |
| Cas9 vectors | ||
| CV01 | Cas9 co-transfection vector (CMV promoter) / 5 ug | $350 |
| CV02 | Cas9/HygR/GFP vector (CMV promoter) / 5 ug | $350 |
| CV03 | Cas9/RFP/PuroR vector (CMV promoter) / 5 ug | $350 |
| CV11 | Cas9 co-transfection vector (EF1a promoter) / 5 ug | $350 |
| CV12 | Cas9/HygR/GFP vector (EF1a promoter) / 5 ug | $350 |
| CV13 | Cas9/RFP/PuroR vector (EF1a promoter) / 5 ug | $350 |