RV01 – pRGS – Reporter with GFP

pRGS is designed as a surrogate reporter system for CRISPR/Cas9 genome editing validation and cell sorting. Featuring a GFP expression cassette in two different reading frame, pRGS enables researchers to monitor transfection efficiency and enrich successfully edited cells using FACS (Fluorescence-Activated Cell Sorting).

The vector facilitates quick screening of genome editing outcomes by coupling GFP expression with nuclease activity. It is compatible with common transfection protocols and ideal to enrich cells with gene editing events.

• GFP-based surrogate reporter plasmid
• Enriches edited cells via FACS
• Monitors transfection and cutting activity
• Compatible with any gene editing system including CRISPR/Cas9 and Cas12a
• Ideal for high-throughput gene editing studies
• Pre-validated for mammalian systems

• Gene editing validation
• Cell sorting with GFP signal
• Functional screening of sgRNAs
• Genome-wide editing enrichment
• Optimization of nuclease delivery systems
• Lab-scale or preclinical gene editing research

• 3 µg pRGS plasmid in TE buffer
• Detailed usage and transfection instructions
• Technical support for CRISPR enrichment protocols

Need assistance with CRISPR enrichment or reporter design? Contact our team for custom vector advice and optimization support.